Ameliorative Role of Virgin Coconut Oil on Tramadol-Induced Nephrotoxicity in Sprague-Dawley Rats

PDF Review History

Published: 2024-04-05

Page: 24-30


O. A. Adebajo

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

P. K. Adebajo

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

O. H. Ayoade

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

U. U. Akpan

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

J. H. Ojo *

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

F. Akande

Anatomy Programme, Bowen University, Iwo, Osun State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

This research aims to investigate the effect of Tramadol induced nephrotoxicity and the ameliorative potency of Virgin Coconut Oil (VCO) on the kidneys using Sprague-Dawley rats as experimental models. A total of 40 rats were used for the study and were grouped 1-4 (130±30g; N=10). Group 1 served as the Control group and was given 1ml of Distilled Water (DW). Groups 2-4 received 25, 50 and 100 mg/kg of Tramadol respectively for 4 weeks. Five animals were randomly selected and euthanized, and the kidneys were harvested for histology and to determine oxidative stress levels, and blood sera were used for kidney function test. The remaining rats in each group received 10 mg/kg of VCO for duration of 2 weeks and were euthanized and above parameters were determined. Results showed an increase in the MDA and decrease in SOD and CAT values in the kidney tissue for the groups administered Tramadol. There was an increase in creatinine values in the groups administered Tramadol and an overall decrease in the values of Urea and Albumin. Sections of the tramadol-treated group showed an area of inflammatory infiltration in the interstitial space while groups administered with VCO showed normal histology. There was an increase in the CAT values for the group treated with VCO. There was an increase in creatinine values in the groups administered VCO and a decrease in the values of urea. In conclusion, Tramadol had adverse effects on the kidney however upon administration of virgin coconut oil (VCO), improvement in the cytoarchitecture of the kidney and levels of the oxidant markers was recorded.

Keywords: Tramadol, virgin coconut oil, creatinine, SOD, CAT, MDA


How to Cite

Adebajo, O. A., Adebajo , P. K., Ayoade , O. H., Akpan, U. U., Ojo , J. H., & Akande , F. (2024). Ameliorative Role of Virgin Coconut Oil on Tramadol-Induced Nephrotoxicity in Sprague-Dawley Rats. Asian Journal of Research in Medicine and Medical Science, 6(1), 24–30. Retrieved from https://jofmedical.com/index.php/AJRMMS/article/view/6

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References

Jamison RN, Mao J. Opioid analgesics in Mayo Clinic Proceedings. Elsevier. 2015;90(7):957-968.

Jatau AI, Sha’aban A, Gulma KA, Shitu Z, Mhalid KG, Isa GM, Wada A, Mustapha AS. The burden of drug abuse in Nigeria: a scoping review of epidemiological studies and drug laws. Public health reviews; 2021.

Subedi M, Bajaj S, Kumar MS, Mayur YC. An overview of tramadol and its usage in pain management and future perspective. Biomedicine & Pharmacotherapy. 2019; 111:443-51.

Bravo L, Mico JA, Berrocoso E. Discovery and development of tramadol for the treatment of pain. Expert Opinion on Drug Discovery. 2017;12(12):1281-91.

Bravo L, Mico JA, Berrocoso E. Discovery and development of tramadol for the treatment of pain. Expert Opinion on Drug Discovery. 2017;12(12):1281-91.

Mike TB, DeVault H, Blackford MG. Acute tramadol ingestion with transient acute kidney injury in an adolescent female. The Journal of Pediatric Pharmacology and Therapeutics. 2021;26(4):411-3.

Jourde-Chiche N, Fakhouri F, Dou L, Bellien J, Burtey S, Frimat M, Jarrot PA, Kaplanski G, Quintrec LM, Pernin V, Rigothier C. Endothelium structure and function in kidney health and disease. Nature Reviews Nephrology. 2019;15(2): 87-108.

Chikezie UE, Ebuenyi ID. Tramadol misuse in the Niger Delta; A review of cases presenting within a year. Journal of Substance Use. 2019;24(5):487-491.

Madukwe AU, Klei A. Tramadol as a pain relieving and physical work performance enhancement medication. Drugs and Alcohol Today. 2020;20(1):27-35.

Yunusa U, Bello UL, Idris M, Haddad MM, Adamu D. Determinants of substance abuse among commercial bus drivers in Kano Metropolis, Kano State, Nigeria. American Journal of Nursing Science. 2017;6(2):125-30.

Ahmad Z, Hasham R, Nor NA, Sarmidi MR. Physico-chemical and antioxidant analysis of virgin coconut oil using West African tall variety. Journal of Advanced Research in Materials Science. 2015;13 (1):1-0.

Narayanankutty A, Illam SP, Raghavamenon AC. Health impacts of different edible oils prepared from coconut (Cocos nucifera): A comprehensive review. Trends in Food Science & Technology. 2018;80:1-7.

Nevin KG, Rajamohan T. Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food chemistry. 2006;99(2):260-266.

Gbotolorun SC, Isah PK, Adebajo OA. Toxicity of Mucuna pruriens seed extract on the kidney of adult Sprague-Dawley rats. African Journal of Pharmacology and Therapeutics. 2018; 7(1).

Mena S, Ortega A, Estrela JM. Oxidative stress in environmental-induced carcinogenesis. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2009;674(1-2):36-44.

Del-Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, metabolism and cardiovascular diseases. 2005;15(4):316-328.

Guéraud F, Taché S, Steghens JP, Milkovic L, Borovic-Sunjic S, Zarkovic N, Gaultier E, Naud N, Héliès-Toussaint C, Pierre F, Priymenko N. Dietary polyunsaturated fatty acids and heme iron induce oxidative stress biomarkers and a cancer promoting environment in the colon of rats. Free Radical Biology and Medicine. 2015;83:192-200.

Bandyopadhyay U, Das D, Banerjee RK. Reactive oxygen species: oxidative damage and pathogenesis. Current Science. 1999;658-66.

Kutlu T, Durmaz G, Ates B, Erdogan A. Protective effect of dietary apricot kernel oil supplementation on cholesterol levels and antioxidant status of liver in hypercholesteremic rats. J Food Agric Environ. 2009;3(4):61-5.

Jaganjac M, Čačev T, Čipak A, Kapitanović S, Trošelj KG, Žarković N. Even stressed cells are individuals: second messengers of free radicals in pathophysiology of cancer. Croatian Medical Journal. 2012;53(4):304-9.

Parlakpinar HA, Tasdemir SE, Polat AL, Bay-Karabulut AY, Vardi NI, Ucar MU, Acet A. Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. Toxicology. 2005;207(2):169-77.

Ahmed MA, Kurkar A. Effects of opioid (tramadol) treatment on testicular functions in adult male rats: The role of nitric oxide and oxidative stress. Clinical and Experimental Pharmacology and Physiology. 2014;41(4):317-23.

Owoade AO, Adetutu A, Olorunnisola OS. Hematological and biochemical changes in blood, liver and kidney tissues under the effect of tramadol treatment. J Alcohol Drug Depend. 2019;7(327):2.

Urbschat A, Obermüller N, Haferkamp A. Biomarkers of kidney injury. Biomarkers. 2011;16(sup1):S22-30.

I.U. Ibrahim. Youth and Drugs: Problems, Consequences and Preventives Measures.